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| Registros recuperados: 273 | |
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| Mingen Xu; Yongnian Yan; Renjie Zhang; Ri Yao; Haixia Liu; Xiaohong Wang. |
| One of the major obstacles in developing multifunctional drugs for the metabolic syndrome (MS) is lack of in vitro models that capture more complex features of the disease. Here we give the first report that establishes an energy metabolic system model using cell-assembly technique which can assemble cells into designated places to form complex three-dimensional structures. Adipose-derived stromal cells were assembled and induced differentiation into adipocytes and endothelial cells; pancreatic islets were then deposited at designated locations and constituted adipoinsular axis with adipocytes. Analysis of the factors involved in energy metabolism showed our system could capture more physiological and pathophysiological features of the in vivo energy... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Pharmacology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/1496/version/1 |
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| Ramar Perumal Samy; S. Ignacimuthu; Vincent TK Chow. |
| Synthetic drugs, which are having serious side effects, are cytotoxic to human beings. Moreover, bacteria slowly become resistant against antibiotics. In order to overcome these problems, scientists are continuing to search for antibacterial and novel principles from plants. Zanthoxylum limonella has been popularly used in traditional systems of medicine for the treatment of dental caries, cardiac, respiratory diseases and stomach problems. The present study was therefore focused on finding out the medicinal properties of the bark of Z. limonella tested against some selected bacteria using disc-diffusion method at the concentration of 100, 200 and 300 mg/ml. The methanol and water extracts of the bark exhibited effective antibacterial activity against S.... |
| Tipo: Manuscript |
Palavras-chave: Microbiology; Pharmacology; Plant Biology. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/6027/version/1 |
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| Sierra Rayne; Kaya Forest; Ken J. Friesen. |
| The pK~a~ estimation ability of the semiempirical PM6 method was evaluated across a broad range of oxyacids and compared to results obtained using the SPARC software program. Compound classes under consideration included acetic acids, alicyclic and aromatic heterocyclic acids, benzoic acids, boronic acids, hydroxamic acids, oximes, peroxides, peroxyacids, phenols, α-saturated acids, α-saturated alcohols, sulfinic acids, α-unsaturated acids, and α-unsaturated alcohols. PM6 accurately predicts the acidity of acetic and benzoic acids and their derivatives, but is less reliable for alicyclic and aromatic heterocyclic acids and phenols. α-Saturated acids are reliably modeled by PM6 except for polyacid... |
| Tipo: Manuscript |
Palavras-chave: Chemistry; Pharmacology; Earth & Environment. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/2981/version/1 |
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| David Basanta; Robert Gatenby; Alexander R. A. Anderson. |
| Although many anti cancer therapies are successful in killing a large percentage of tumour cells when initially administered, the evolutionary dynamics underpinning tumour progression mean that often resistance is an inevitable outcome, allowing for new tumour phenotypes to emerge that are unhindered by the therapy. Research in the field of ecology suggests that an evolutionary double bind could be an effective way to treat tumours. In an evolutionary double bind two therapies are used in combination such that evolving resistance to one leaves individuals more susceptible to the other. In this paper we present a general evolutionary game theory model of a double bind to study the effect that such approach would have in cancer. Furthermore we use this... |
| Tipo: Manuscript |
Palavras-chave: Cancer; Ecology; Pharmacology; Evolutionary Biology. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/6380/version/1 |
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| C. S. Lin; Jhen Ru Shih; C. H. Hsu; Z.H. Wen; Chiranjib Chakraborty. |
| Expression of the target gene is important for gene therapy. Presently, localized transgenesis is used for gene therapy which can be achieved by a target gene expression. Here, we have reported the plasmid mediated gene therapy to zebrafish model. For this purpose, we have chosen green fluorescent protein (GFP) as a target gene because the expression can be detected easily. GFP was inserted in a plasmid vector, pQE30 to develop the vector pQE30GFP. The plasmid pQE30GFP was constructed form plasmid, pQE30 and pEGFPC2. pQE30GFP injected directly in one group of fish into the muscle where luciferase expression was noted. In another group, after injection electroporation was performed where we have also noted luciferase expression; but, electroporation cause... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Genetics & Genomics; Pharmacology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/2718/version/1 |
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| Suaib Luqman. |
| Moringa oleifera of family Moringaceae, commonly known as Horseradish-tree or the Ben-oil tree is an exceptionally nutritious vegetable tree with a variety of medicinal uses, distributed in tropical and subtropical regions of the world. The tree's bark, roots, fruit (pod), flowers, leaves, seeds and gum are used as an antiseptic, anticancer, anti-inflammatory, hepatoprotective and in treating rheumatism, venomous bites and other conditions. The immature green pods, called ‘drumsticks’ are probably the most valued and widely used part of the tree for water purification (e.g. desalination of ocean salt water). The leaves are highly nutritious, being a significant source of beta-carotene, vitamin C, protein, iron and potassium... |
| Tipo: Presentation |
Palavras-chave: Molecular Cell Biology; Pharmacology. |
| Ano: 2012 |
URL: http://precedings.nature.com/documents/6924/version/1 |
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| Christoph Jung; Sigrun Polier; Markus Schoeffel; M Drechsler; Valerie Jerome; Ruth Freitag. |
| The delivery of drugs to cells is a very active area of research. Drugs are commonly believed to ameliorate illnesses. The targeted, controlled and/or enhanced uptake of the drug into cells is facilitated by loading the drug into carrier vehicles (nanoparticles, e.g.. micelles).The distribution of a drug over the nanoparticles used to deliver the drug to the targeted cells is of vital importance. It makes a difference, whether all nanoparticles carry the same amount of drug or whether a little amount of the nanoparticles carries a high proportion of the drug material and most nanoparticles carry no material. This depends on the method used to load the drugs into the carriers. Furthermore, the absolute amount of drug present in the nanoparticles and the... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Pharmacology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4140/version/1 |
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| Mostafa Saffari; Malihe Shahbazi; Mehdi Shafiee Ardestani. |
| The aim of this study was to formulate and evaluate controlled release preparations of a water-insoluble drug using Copolymers synthesized from acrylic and methacrylic acid
esters (Eudragit L 100) as the retardant material. Microspheres were prepared by solvent evaporation method using an alcohol / liquid paraffin system. Span60 was used as the droplet stabilizer. The prepared microspheres were characterized for their micromeritic properties and drug loading, as well as by differential scanning calorimetry. The in vitro release studies were performed first in pH1.2 and then pH 6.8, phosphate buffer. The prepared microspheres were yellow, and spherical in shape. The drugloaded microspheres showed 71-85% of entrapment and release was... |
| Tipo: Manuscript |
Palavras-chave: Chemistry; Pharmacology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/1544/version/1 |
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| Vladimir L. Katanaev; Silke Buestorf. |
| Receptors of the Frizzled family initiate Wnt ligand-dependent signaling controlling
multiple steps in organism development and highly conserved in evolution.
Misactivation of the Wnt/Frizzled signaling is cancerogenic. Frizzled receptors
launch several signaling cascades: the canonical pathway regulating beta-catenin-dependent transcription; the planar cell polarity pathway polarizing the
cytoskeleton within the epithelial plane; and the calcium pathway. Frizzled
receptors possess seven transmembrane domains and their signaling depends on
trimeric G proteins in various organisms. However, Frizzleds constitute a
distinct group within the G... |
| Tipo: Manuscript |
Palavras-chave: Cancer; Developmental Biology; Molecular Cell Biology; Pharmacology. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/2765/version/1 |
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| Abhay Sharma; Farhan Mohammad; Priyanka Singh. |
| A male Drosophila model of locomotor deficit induced by chronic pentylenetetrazole (PTZ), a proconvulsant used to model epileptogenesis in rodents, has recently been described. Antiepileptic drugs (AEDs) ameliorate development of this behavioral abnormality. Time-series of microarray profiling of heads of male flies treated with PTZ has shown epileptogenesis-like transcriptomic perturbation in the fly model. Gender differences are known to exist in neurological and psychiatric conditions including epileptogenesis. We describe here the effects of chronic PTZ in Drosophila females, and compare the results with the male model. As in males, chronic PTZ was found found to cause a decreased climbing speed in females. In males, overrepresentation of Wnt, MAPK,... |
| Tipo: Manuscript |
Palavras-chave: Genetics & Genomics; Neuroscience; Pharmacology; Bioinformatics. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/3460/version/1 |
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| Reda Mahfouz; Ania Jankowska; Quteba Ebrahem; Zhenbo Hu; Pramod Terse; Joseph Covey; Kenneth Chan; Yonghua Ling; Kory Engelke; Mikkael Sekeres; Ramon Tiu; Jaroslaw Maciejewski; Tomas Radivoyevitch; Yogen Saunthararajah. |
| In vivo, half-lives of cytidine analogues such as 5-azacytidine and decitabine, used to treat myelodysplastic syndromes (MDS), are determined largely by cytidine deaminase (CDA), an enzyme that rapidly metabolizes these drugs into inactive uridine counterparts. Genetic factors influence CDA activity, and hence, could impact 5-azacytidine/decitabine levels and efficacy, a possibility requiring evaluation. Using an HPLC assay, plasma CDA activity was confirmed to be decreased in individuals with the CDA SNP A79C. More interestingly, there was an even larger decrease in females. Explaining the decrease in enzyme activity, liver CDA expression was significantly lower in female versus male mice. As expected, decitabine plasma levels, measured by... |
| Tipo: Manuscript |
Palavras-chave: Cancer; Genetics & Genomics; Pharmacology. |
| Ano: 2012 |
URL: http://precedings.nature.com/documents/6971/version/1 |
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| Manuela G. Neuman; Radu M. Nanau; Lawrence Cohen; Paul A. Hwang. |
| Hypersensitivity syndrome reactions (HSR) to antiepileptic drugs (AED) are associated with severe clinical cutaneous adverse reactions (SCAR).Our aims are: to assess HSRs to AEDs using the in vitro lymphocyte toxicity assay (LTA) in patients who manifested HSRs clinically, to correlate LTA results with the clinical syndrome, to correlate LTA results with the human leukocyte antigen (HLA) allele B*1502 (HLA-B*1502) positivity in a Han Chinese-Canadian population, and to determine the cytokine network in this population. HSR patients developed fever and cutaneous eruptions in the presence or absence of organ involvement within 8 weeks of exposure to carbamazepine (CBZ), phenytoin (PHY) or lamotrigine (LTG). Control patients received AEDs without presenting... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Genetics & Genomics; Immunology; Pharmacology. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/6477/version/1 |
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| Jayapal J. Manikandan; Alirio Jose P. N. Melendez; Peter Natesan A. J. Pushparaj. |
| Mast cell activation, mediates type-1 allergic responses, one of the most powerful reactions of the immune system. However, mast cells activation is becoming increasingly linked to inflammatory, autoimmunity, and to adaptive immunity by regulating T-cell activation.
Here we analyzed the gene expression pattern in IgE-sensitized and FcεRI aggregation on human mast cells. Our data revealed coordinated changes in gene expression. We observed increased expression of gene-transcripts involved in allergic, innate and adaptive
immune responses. Among the most prominent findings is the increased expression of transcripts encoding for MIP3a, SPARCL1, AREG, IL18, CCL1, TNFRSF9, IL1b, CX3CR1, PTGER3, MIF, MMP12, ADORA3,... |
| Tipo: Poster |
Palavras-chave: Immunology; Molecular Cell Biology; Pharmacology; Bioinformatics. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/1150/version/1 |
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| Awadh Bihari Yadav; Amit K. Singh; Amit Misra. |
| Targeted drug delivery to alveolar macrophages harboring Mycobacterium tuberculosis (Mtb) holds promise of high efficacy against pulmonary tuberculosis (TB). It was investigated whether inhalable microparticles (MP) can rescue macrophages from ‘alternative’ activation induced by pathogenic Mtb in addition to achieving targeted drug delivery. A genome-wide transcription analysis (Affymetrix HG-U133 Plus 2.0 DNA microarray) of THP-1 cell line derived macrophages was undertaken after exposing them to infection with 10 MOI of MTB H37Rv at 0, 12 and 24 hours post infection. The Molecular markers of macrophage bactericidal activity were assayed in THP-1- and primary human peripheral blood mononuclear cell (PBMC)-derived macrophages, in the... |
| Tipo: Poster |
Palavras-chave: Pharmacology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/2453/version/1 |
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| Chao Liu; Jing Guan; Ying Chen; Kunshen Liu. |
| Glucocorticoids, produced in adrenal cortex, exert potent natriuretic and diuretic actions in the kidney. Recently, it has been found that glucocorticoids could upregulate the expression of natriuretic peptide receptor A (NPR-A), the primary receptor of atrial natriuretic peptide, in the hypothalamus of the rat. Consequently, systemic administration of glucocorticoid could block dehydration-induced water intake by activation hypothalamic NPR-A. We describe here glucocorticoids could inhibit sodium intake when administrated systemically in conscious, salt-depleted rats; an effect which was strong and long-lasting. The study provided further evidence for the actions of glucocorticoids on central nervous system, which together with their established renal... |
| Tipo: Manuscript |
Palavras-chave: Neuroscience; Pharmacology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/5376/version/1 |
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| Zhidong Zhou; Tit Meng Lim. |
| In this study we demonstrate for the first time that GSH could rapidly conjugate with dopamine (DA)-derived DA-o-quinones without enzymatic catalysis to form short-lived intermediate GSH-conjugates (2-S-GSH-DA-o-quinone and 5-S-GSH-DA-o-quinone). These intermediate GSH-conjugates are unstable and would finally form reactive or non-reactive GSH-conjugates dependent on ambient reductive forces. Under insufficient reductive forces, the intermediate GSH-conjugates could cyclize spontaneously to form reactive 7-S-GSH-aminochrome (7-S-GSH-AM). The 7-S-GSH-AM is so reactive that it could further react with another GSH to form 4,7-bi-GSH-5,6-dihydroindole. Its reactivity could also abrogate tyrosinase activity in solutions. In addition, the 7-S-GSH-AM could... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Chemistry; Neuroscience; Pharmacology. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/2963/version/1 |
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| Registros recuperados: 273 | |
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